Mothur is an opensource, expandable bioinformatics software for the microbial ecology community.

There are two installations of mothur, both are of version 1.39.5. One is the MPI-enabled version and another is a non-MPI version. If you just need to use a single compute node, you will want to use the non-MPI version; for multi-node parallelization (more than 32 processes), you will want to use the MPI version. Using the MPI version for a single node multi-threading parallelization will result in slower performance.

To use mothur, you can use the module command.

For non-MPI version:

module load mothur/1.39.5

For MPI version, you also need to load openmpi:

module load mothur/1.39.5_mpi openmpi/gnu/2.0.2

Then you can start Mothur typing:

mothur

You can either run mothur interactively on compute nodes or submit the calculation to the compute nodes.